Fig. 1: Imaging Mass Cytometry (IMC) Profiling of Self-Reported Black American (BA) and White American (WA) Triple Negative Breast Cancer (TNBC). | Nature Communications

Fig. 1: Imaging Mass Cytometry (IMC) Profiling of Self-Reported Black American (BA) and White American (WA) Triple Negative Breast Cancer (TNBC).

From: Integrative spatial omics reveals distinct tumor-promoting multicellular niches and immunosuppressive mechanisms in Black American and White American patients with TNBC

Fig. 1

a Schematic representation of the clinical cohorts analyzed, and the corresponding types of analyses performed for each group. For the Baylor Scott & White (BSW)2 validation cohort sample numbers 10/9 describe samples examined using imaging mass cytometry and 10X spatial transcriptomics, respectively. b Overview of the analytical workflow employed throughout the study. c Diagram illustrating the imaging mass cytometry-based immune cell profiling process applied to BA and WA TNBC tumors, utilizing a tissue microarray (TMA) format. ROI: Region Of Interest. Figure created in BioRender. Sreekumar, A. (2025) https://BioRender.com/c0tobe3. d Unsupervised clustering of individual cells, segmented from IMC data of BA and WA TNBC tumors, identified 20 distinct clusters. The differential protein markers and their corresponding nomenclature (columns) across these 20 clusters (rows) are shown. Source data are provided in Github (10.5281/zenodo.15353111). e Representative t-SNE plots illustrating the distribution of the 20 clusters as described in panel (d) within the expression space. f Overlay of expression patterns for E-Cadherin, CD45RA, CD31, CD68, and Vimentin onto the t-SNE plots.

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