Fig. 4: Identification and characterization of spider nrEVEs and related transcripts in different individual. | Nature Communications

Fig. 4: Identification and characterization of spider nrEVEs and related transcripts in different individual.

From: Discovery of novel non-retroviral endogenous viral elements reveals their long-term integration history in spiders

Fig. 4

a This scheme illustrates the relationship between the number of datasets and the identified number of expressed baculoviral homologs (BHs) in different Araneae families from TSA databases. The color of the points represented the number of homologs of 22 distinct baculoviral core proteins in scaffolds. b The size and strand distribution of small RNAs targeting BHs in three parts of major silk gland of available T. calvata sRNA sequencing data is depicted. The localization of BHs on chromosomes is labeled as a reference. c The Ka/Ks analysis of top nine scaffolds containing the highest number of distinct homologs of baculoviral core proteins (AvEVE1-9). The Ka/Ks analysis of AvEVE1-9 was carried out on the intact ORFs between our sequencing data generated consensus sequences and the reference genome assembly of WGS database (BGPR01). d The qRT-PCR result of BHs of collected female T. clavata samples (n = 3) in different tissues. The expression of BHs was first normalized with 16S rRNA and then compared to the corresponding expression levels of leg. Data represent mean ± s.e.m. Statistical analysis was performed using repeated-measures two-way ANOVA with multiple comparisons and Tukey’s correction. The source data and P values were provided in Source Data file.

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