Fig. 2: IgG1 and IgG4 antibody responses to Opisthorchis viverrini recombinant proteins printed on a proteome microarray. | Nature Communications

Fig. 2: IgG1 and IgG4 antibody responses to Opisthorchis viverrini recombinant proteins printed on a proteome microarray.

From: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma

Fig. 2

Top 40 protein hits ranked by t-test significance comparing IgG1 and IgG4 (a) responses from 100 negative subjects (50 O. viverrini fecal egg count test (FECT)-negative subjects living in an endemic region of Thailand and 50 non-endemic negative subjects residing in the US, red bars) and 50 O. viverrini FECT-positive subjects (Thailand, blue/white bars). RFU: relative fluorescence units. Bars indicate standard error of the mean (SEM). p-value comparing infected and uninfected negative control responses shown as green dots with green dashed line marking the p < 0.05 threshold. Protein accession numbers are provided on the y axis, and fluke extracts (OvES, excretory/secretory antigen; OvSo, somatic lysate antigen) were printed as controls. Immunoreactive proteins selected from the proteome array screen (b) for the serodiagnosis of opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma (CCA). Protein accession codes and biological functions (families) are listed, and for ease of nomenclature designated as P1-P9. The heatmap reflects the degree of statistical significance for IgG1 or IgG4 antibody levels (relative fluorescence units) in O. viverrini FECT positive subjects (Ov + ), Clonorchis sinensis FECT positive subjects (Cs + ) or CCA patients compared to healthy controls (both endemic FECT-negative and US donors). Significance values < 0.05 are denoted by colour, scaling from least significant (yellow) to most significant (purple). “X” denotes p > 0.05 or where the healthy control signal was higher than the disease groups.

Back to article page