Fig. 7: Retinoic acid regulates pioneer neuron axon targeting.
From: Axon targeting of transcriptionally distinct pioneer neurons is regulated by retinoic acid signaling
A Schematic of mosaic labeling strategy. Tg(hsp70l:mCherry, en.silll)nl30 embryos were injected with one of the following plasmids: SILL:EGFP-CAAX, SILL:caRAR-CAAX, or SILL:dnRAR-CAAX at the one-cell stage. Animals were grown to 3 days and assessed for the presence of single EGFP-positive neurons in the pLL ganglion and the location of EGFP-positive axon terminals. Created in BioRender. Lab, N. (2025) https://BioRender.com/vij5z6b. B–G Representative confocal images of single labeled neurons in the pLLg and their targets following overexpression of EGFP (B, E), caRAR (C, F), or dnRAR (D, G). The following number of cells were examined in 3 independent experiments (1 cell/animal): EGFP – 34; caRAR – 31, and dnRAR – 14. H Frequency of neuromast targeting along zebrafish trunk by neurons labeled with EGFP, caRAR, or dnRAR. Difference in axon targeting assessed by Chi-Square: EGFP vs. caRAR p = 0.0011; EGFP vs. dnRAR p = 0.0795; caRAR vs dnRAR p = 0.0001. All images are lateral views with anterior to left. Scale bars = 20 μm. All images scaled the same.
