Fig. 1: Effects of a fiber diet on physiological parameters in 2-month-old male mice.

a Outline of feeding regimens (CR, RD, IF10, IF 20, IF30, IF30m, CF10, CF20, CF30 and CF30m) during day 1 to 13. ZT: Zeitgeber Time, refers to the light exposure. Food and water consumption were measured at ZT12. b Experimental design showing the 4-h food intake test (except for the CR group) on day 0 and day 14. Food and water consumption were measured hourly. Trend in food intake for inulin (c) or cellulose (d) fiber diet groups from day 1 to 13. e Average food intake of all groups from day 6 to 13. Every point is the daily food intake of mice in each group from day 6 to day 13, a total of 8 points. Body weight of mice during the two-week inulin (f) or cellulose (g) fiber diet intervention. h Average body weight of mice in all ten groups. Every point is the average body weight of mice in each group from day 1 to day 14, a total of 14 points. i Diet composition of eight redesigned fiber diets based on the AIN-93G formulation. Average protein (j), carbohydrate (k), or calorie intake (l) of mice from day 6 to 13. Every point is the average measurement data of 6 mice from day 6 to 13, a total of 8 points. Data are presented as mean ± SEM, n = 6 mice per group. Statistical significance was determined by one-way ANOVA (Dunnett’s test). *P < 0.05, **P < 0.01, ***P < 0.001.