Fig. 2: A night 2 h feeding regimen mimics the transcriptional patterns of CR in young male mice.
From: A high-fiber diet mimics aging-related signatures of caloric restriction in mammals
a Volcano plots showing differentially expressed genes (DEGs) in liver of CR versus RD mice. Red denotes genes represent significantly upregulated genes in CR mice (1472 genes); blue denotes represent significantly downregulated genes in CR mice (2191 genes). DEGs were identified with FDR < 0.05. Top 20 GO terms enriched in genes upregulated (b) or downregulated (c) by CR. d Venn diagram (top) showing the overlap of CR/RD upregulated genes in 3-month-old mice (this study) with CR-night−2h/AL up-regulated genes in 6-month-old mice20, and the pathway enrichment results of overlapping genes (bottom). e Venn diagram (top) showing the overlap of CR/RD downregulated genes in 3-month-old mice with CR-night-2h/AL downregulated genes in 6-month-old mice, and the pathway enrichment results of overlapping genes (bottom). f CR-mediated fold change (FC) for overlapping genes across three age stages. g Venn diagram (top) showing the overlap between CR up/downregulated genes and rhythmic genes in young (6-month-old) or old (19-month-old) animals, heatmap (bottom) showing expression FC for the overlapping rhythmic genes across three age stages. The sets of rhythmic genes were identified from a previous study20. h Venn diagram (top) showing the overlap among CR up/downregulated genes, rhythmic genes, and inflammatory response genes. Heatmap (bottom) showing expression FC for overlapping inflammatory response genes across three age stages. All Inflammatory response genes were from GO term inflammatory response (GO:0006954). i Venn diagram (top) showing the overlap among CR-regulated genes, aging related genes, and CR-night-2h/AL (6 months) DEGs. Heatmap (bottom) showing expression fold change (FC) for collagen genes across three age stages.
