Fig. 1: Specific nutrients dissociate capsular polysaccharide (CPS) from mucoidy.
From: Arginine regulates the mucoid phenotype of hypervirulent Klebsiella pneumoniae
Wild-type K. pneumoniae strain KPPR1 was cultured in low-iron M9 minimal medium with 20 mM glucose (Glc), 20 mM glycerol (Glyc), or 20 mM fucose (Fuc), with or without 1% casamino acids (CAA), as nutrient sources. A Mucoidy was determined by quantifying the supernatant OD600 after sedimenting 0.5 OD600 unit of culture at 1000 x g for 5 min. B Uronic acid content of crude CPS extracts were quantified and normalized to the OD600 of the overnight culture. Data presented are the mean, and error bars represent the standard error of the mean. Statistical significance was determined using a one-way ANOVA with a Tukey post-test. p-values are displayed above each comparison, # p < 0.0001. The statistics displayed above the data bars represent values relative to M9 + CAA. Statistics connected by lines identify other direct comparisons. Exact P-values: (A) left to right above data bars: <0.0001, <0.0001, <0.0001, <0.0001, <0.0001; connected by lines: <0.0001, <0.0001, <0.0001; (B) left to right: <0.0001. Experiments were performed 3 independent times, each in biological triplicate (n = 9). Source data are provided as a Source Data file.
