Table 1 Primer sequences qRT-PCR
Gene | Forward Primer | Reverse Primer |
|---|---|---|
Human primers | ||
ACTA2 | ACCCGATAGAACATGGCATCA | GGCAACACGAAGCTCATTGTA |
ALPL | ACTGGTACTCAGACAACGAGAT | ACGTCAATGTCCCTGATGTTATG |
ANKRD1 | GATCGAATTCCGTGATATGCT | AAACATCCAGGTTTCCTCCA |
BCL2 | GGTGGGGTCATGTGTGTGG | CGGTTCAGGTACTCAGTCATCC |
BDNF | GGCTTGACATCATTGGCTGAC | CATTGGGCCGAACTTTCTGGT |
BGN | GAGACCCTGAATGAACTCCACC | CTCCCGTTCTCGATCATCCTG |
BIRC5 | AGGACCACCGCATCTCTACAT | AAGTCTGGCTCGTTCTCAGTG |
CCL2 | CAGCCAGATGCAATCAATGCC | TGGAATCCTGAACCCACTTCT |
CCL3 | GCAACCAGTTCTCTGCATCA | AAGATGACACCGGGCTTG |
CCL5 | CCAGCAGTCGTCTTTGTCAC | CTCTGGGTTGGCACACACTT |
CCN2 | GCCTCCTGCAGGCTAGAGA | GATGCACTTTTTGCCCTTCT |
CCNA2 | CCATACCTCAAGTATTTGCCATC | TCCAGTCTTTCGTATTAATGATT |
CCND1 | CAATGACCCCGCACGATTTC | CATGGAGGGCGGATTGGAA |
CD68 | GGAAATGCCACGGTTCATCCA | TGGGGTTCAGTACAGAGATGC |
CREM | ACACCACCTAGTATTGCTACCA | GGATTGTTCCACCTTGGGCTAT |
CYR61 | TCCAGGGCACACCTAGACA | GCCCATTTTCTCCATGATTC |
FGF2 | AGTGTGTGCTAACCGTTACCT | ACTGCCCAGTTCGTTTCAGTG |
GAPDH | GAACGGGAAGCTTGTCATCAA | ATCGCCCCACTTGATTTTGG |
GNAS | TGCCTCGGGAACAGTAAGAC | GCCGCCCTCTCCATTAAAC |
GPR153 | CGTGTCCACCTTCTACACCC | GCTCAGCCGGTAGTTGACAG |
ICAM1 | CCCCTACCAGCTCCAGACC | TGCGTGTCCACCTCTAGGAC |
IL1b | ATGATGGCTTATTACAGTGGCAA | GTCGGAGATTCGTAGCTGGA |
IL6 | AGAGCTGTGCAGATGAGTACAA | GTTGGGTCAGGGGTGGTTA |
ITGAM | ACTTGCAGTGAGAACACGTATG | TCATCCGCCGAAAGTCATGTG |
LUM | GGATTGGTAAACCTGACCTTCAT | GATAAACGCAGATACTGCAATGC |
MKI67 | CAGAGAATTTGCTTGGAAAACA | GAGGTGGGGAGCAGAGGT |
MYC | GGCTCCTGGCAAAAGGTCA | CTGCGTAGTTGTGCTGATGT |
MYH11 | CTGCAGCAGCTCTTCAACC | CCTCGCGCTGGTACTCCT |
NR4A1 | ATGCCCTGTATCCAAGCCC | GTGTAGCCGTCCATGAAGGT |
PCNA | CCTAAAGATTCTGAAAAAGAGAA | CAGGTTGCAAAGGACATGC |
PDGFB | CTCGATCCGCTCCTTTGATGA | CGTTGGTGCGGTCTATGAG |
PTGS2 | CTGGCGCTCAGCCATACAG | CGCACTTATACTGGTCAAATCCC |
RUNX2 | TCAACGATCTGAGATTTGTGGG | GGGGAGGATTTGTGAAGACGG |
SPP1 | GAAGTTTCGCAGACCTGACAT | GTATGCACCATTCAACTCCTCG |
TAGLN | GGCCAAGGCTCTACTGTCTG | GCCATAGGAAGGACCCTTGT |
TNF | CCCTGGTATGAGCCCATCT | CTGAGTCGGTCACCCTTCTC |
VCAM1 | CAGGCTGGAAGAAGCAGAAA | CACTCTCAGAAGGAAAAGCTGTA |
VEGFA | AGGGCAGAATCATCACGAAGT | AGGGTCTCGATTGGATGGCA |
Murine primers | ||
Acta2 | GAGGCACCACTGAACCCTAA | TACATGGCGGGGACATTGAA |
Actb | CCCTAAGGCCAACCGTGAAA | CAGCCTGGATGGCTACGTAC |
Ccl2 | CATCCACGTGTTGGCTTA | GATCATCTTGCTGGTGAATGAGT |
Ccl5 | GCTGCTTTGCCTACCTCTCC | TCGAGTGACAAACACGACTGC |
Cdh5 | AACGAGGACAGCAACTTCAC | TGGCATGCTCCCGATTAAAC |
Fgf2 | CGGCTCTACTGCAAGAACG | TGCTTGGAGTTGTAGTTTGACG |
Gapdh | AGCTTGTCATCAACGGGAAG | TTTGATGTTAGTGGGGTCTCG |
Gpr153 | CGATGGCCAGTCAACTACC | CCGACAGGATGAAGGACAC |
Icam1 | AGGGCTGGCATTGTTCTCTA | TGTCGAGCTTTGGGATGGTA |
Il-6 | CGATGATGCACTTGCAGAAA | ACTCCAGAAGACCAGAGGAA |
Mki67 | GAGACATACCTGAGCCCATCA | GCTTTGCTGCATTCCGAGTA |
Myh11 | AAGCTGCGGCTAGAGGTCA | CCCTCCCTTTGATGGCTGAG |
Pcna | TTTGAGGCACGCCTGATCC | GGAGACGTGAGACGAGTCCAT |
Tagln | ACCAAAAACGATGGAAACTACCG | GTGAAGTCCCTCTTATGCTCCT |
Tgfb1 | TGGAGCAACATGTGGAACTC | CAGCAGCCGGTTACCAAG |
Tnf | GGGTGATCGGTCCCCAAA | TGAGGGTCTGGGCCATAGAA |
Vcam1 | CCCAAACAGAGGCAGAGTGTA | CTGCAGGATGCATTGGTACAC |
