Fig. 4: Broad-spectrum antiviral activity of Jun6504 against enteroviruses.
From: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
a Antiviral activity of Jun6504 against five contemporary EV-D68 strains in RD cells. EC50 values against EV-D68 strains were determined using the cytopathic effect (CPE) assay in RD cells after 60 h of compound incubation. The EC50 values are the mean ± SD of three biological replicates. b Antiviral activity of Jun6504 against poliovirus, CVA16, CVA6, and CVB3 in CPE assay. The EC50 values are the mean ± SD of two biological replicates. c Antiviral activity of Jun6504 against EV-A71 and EV-D68 in plaque assay. The EC50 values are the mean ± SD of two biological replicates. d Cytotoxicity of Jun6504 in HeLa, RD, and Vero cells. The results are from two biological replicates. e DSF assay of Jun6504 with EV-A71, EV-D68, and CVB3 2 C proteins. The results are from two biological replicates. f Jun6504 time-of-addition experiment in a single cycle of EV-D68 replication. g Viral titers in the time-of-addition experiment. EC50 and CC50 curve fittings using log10 (concentration of Jun6504) vs. percentage of positive control with variable slopes were performed in Prism 8. Error bars denote mean ± SD of two biological replicates. Source data are provided as a Source Data file.
