Fig. 3: Architecture of the RNA-bound SLFN14 complex.
From: CryoEM structure of the SLFN14 endoribonuclease reveals insight into RNA binding and cleavage
a Orthogonal views of state 1 SLFN14•RNA cryoEM reconstruction shown in blue (NTD), yellow (MD), and brown (CTD). Density within the RNA cleft is shown in pink (RNA) and gray (unassigned). The dimeric RNA-bound SLFN14 protomers are distinguished by dark and light colors as well as the absence and presence of a prime symbol. General dimensions of the SLFN14 reconstruction are reported in Å. b Model of dimeric SLFN14•RNA colored as seen in (panel a). Mg2+ and Zn2+ metal ion sites are circled. c Inset of the SLFN14•RNA cleft. SLFN14 surface representation and cartoon of select N-lobe and C-lobe residues along the RNA interfaces. d 2D diagram of the SLFN14 N- and C-lobe RNA interfaces, where residues are colored based on Consurf sequence conservation83.
