Fig. 1: Quantitative proteomics identifies interactors of ADPr-Ub probe 1.
From: Identification of RNF114 as ADPr-Ub reader through non-hydrolysable ubiquitinated ADP-ribose
a Schematic representation of the ubiquitin conjugation activity of DTX enzymes on ADP-ribosylated substrates. b Chemical synthesis scheme to obtain 1 via CuAAC between 3’-O-propargylated ADPr and bt-Ub bearing a C-terminal azide. c Schematic representation of the workflow for quantitative proteomics to identify proteins interacting with ADPr-Ub probe 1. d Volcano plot showing the interactome of probe 1. Data are normalized to the Ub75 interactome. Dotted lines represent significance and correspond to the thresholds: log2 ratio ≥ 1.3; p-value ≤ 0.05. A two-sided student’s t test with permutation-based FDR was used to calculate the significance between probe and control at 0.05 FDR (p-value). Source data are provided as a Source Data file.
