Fig. 3: RNF114 elongates Ub chain on ADPr-Ub.
From: Identification of RNF114 as ADPr-Ub reader through non-hydrolysable ubiquitinated ADP-ribose
a 32P-NAD + -Ub formation assay with DTX2-RD and RNF114. RNF114 elongates Deltex-catalysed 32P-NAD+-Ub. b Ubiquitination assay using biotin-Ub and biotin-ADPr-Ub probe 1. RNF114 elongates the Ub chain on biotin-Ub and biotin-ADPr-Ub probe 1. c RNF114 elongates Ub chains in a time-dependent manner. Biotin signal is shown in green, and ubiquitin signal is shown in red. d Zoom-in on the decreasing levels of biotin-ADPr-Ub or biotin-Ub (top) and quantification of the remaining biotin signal (bottom). a–d All in the presence of E1/UbcH5B/Ub/Mg2+-ATP. Data are presented as mean values ± SEM. For all experiments, two independent biological replicates were performed with similar results. Source data are provided as a Source Data file.
