Fig. 6: RNF114 recruitment to sites of DNA damage is dependent on its tandem ZnF2 + ZnF3 + UIM domains.
From: Identification of RNF114 as ADPr-Ub reader through non-hydrolysable ubiquitinated ADP-ribose
a Representative images of YFP-RNF114 FL or truncations and mutants recruitment to sites of DNA damage induced by 405 nm irradiation in U2OS WT cells. Arrow heads indicate the site of damage. Scale bar, 5 µm. b Recruitment kinetics of YFP-RNF114 WT (Black), UIM mutant (Blue), ΔUIM (Red), C176A (green) or Y186A (Orange) to sites of damage. c Representative images of YFP-RNF114 ZnF2 + 3 + UIM WT or mutants recruitment to sites of DNA damage induced by 405 nm irradiation in U2OS WT cells. Arrow heads indicate the site of damage. Scale bar, 5 µm. d Recruitment kinetics of YFP-RNF114 ZnF2 + 3 + UIM (Black), UIM mutant (Blue), C176A (green) or Y186A (Orange) to sites of damage. Data are representative of three independent biological replicates. Curves show mean ± s.e.m. of at least 14 cells per condition. Source data are provided as a Source Data file.
