Fig. 3: Carbon source optimization for recombinant protein production in K.phaffii. | Nature Communications

Fig. 3: Carbon source optimization for recombinant protein production in K.phaffii.

From: Accelerating cell culture media development using Bayesian optimization-based iterative experimental design

Fig. 3

A Workflow and study parameters for optimizing media supplements to maximize recombinant protein production in cultures of K.phaffii. B Comparison of specific productivity using optimized media compositions and 2 benchmark conditions for RBDJ, HSA, and trastuzumab. Individual double-sided t-test was performed between the biological replicate experiments performed with benchmark media and optimal media formulation. *, **, *** signify a p-value < 0.1, 0.05, 0.01, respectively with the exact values reported in the Source Data.xlsx file. C Optimized compositions of carbon sources and specific productivities for the respective proteins. D Comparison of the number of experiments to execute the different strategies for designing experiments. Source data are provided in Source Data.xlsx file. BO Bayesian Optimization, OFAT One Factor At Time, BBD Box Behnken Design, CCD Central Composite Design, FF Full factorial, FracFact Fractional Factorial.

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