Fig. 3: Kitlo HSCs exhibit distinct epigenetic features that facilitate lymphoid potential. | Nature Communications

Fig. 3: Kitlo HSCs exhibit distinct epigenetic features that facilitate lymphoid potential.

From: Kitlo hematopoietic stem cells exhibit distinct lymphoid-primed chromatin landscapes that enhance thymic reconstitution

Fig. 3

A UMAP of 6 unsupervised HSC clusters identified by tile-based scATAC-seq analysis after Harmony batch correction. B–D Annotated by age (B), HSC subsets (C), and Kit subsets (D) on the scATAC-seq UMAP. E scATAC-seq cluster composition by HSC subsets (left) and Kit subsets (right). Color scale denotes correlation values between the ATAC clusters and cell annotations, generated using ArchR. F, G Matrix plots showing motif enrichment identified by ChromVAR (F) and gene accessibility (G) for lymphoid-specifying transcription factors for Kit subsets in young and old HSCs. Z-scores denote chromVAR motif enrichment in (F) and gene accessibility scores (peak matrix values) that have been row-scaled in (G), generated using ArchR.

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