Fig. 1: MPXV has a broad cellular tropism in hNOs and 2D neural cultures.
From: Monkeypox virus spreads from cell-to-cell and leads to neuronal death in human neural organoids
a Representative micrographs showing the cell diversity and morphology of ESC-derived hNOs at selected developmental time points (10, 60, and 112 days). Selected markers of NPCs (SOX2, pink) and neurons (TUJ1, orange) are presented. DAPI, blue. Scale bar, 50 µm. b Representative micrographs illustrating the target cells of MPXV in 70-75 days old hNOs 10 days p.i. with a clade IIb MPXV isolate at an MOI of 0.1 TCID50/cell. DAPI, blue; MPXV, green; SOX2, pink; TUJ1, orange. Scale bar, 10 µm. Characterization of 2D NPC (c) and forebrain neuron (d) cultures. DAPI, blue; SOX2, pink; TUJ1, orange. Scale bar, 100 μm. Exemplary image of 2D-cultured NPCs (e) and neurons (f) 8 h p.i. with a clade IIb MPXV isolate at an MOI of 0.01 TCID50/cell. DAPI, blue; MPXV, green; SOX2, pink; TUJ1, orange. Scale bar, 10 μm.
