Fig. 2: Productive MPXV infection in hNOs and 2D cultured neural cells.
From: Monkeypox virus spreads from cell-to-cell and leads to neuronal death in human neural organoids
a Representative micrographs of hNOs infected with a clade IIb MPXV isolate at a rate of 0.1 TCID50/cell at 83 days of development. Infection was followed by immunofluorescence from 2 to 14 days p.i. by imaging of sectioned hNOs. DAPI, blue; MPXV, green. Scale bar, 1000 µm. b Representative micrographs depicting infection progression of 2D cultured NPCs challenged with an mNG-expressing clade IIb MPXV reporter construct at a rate of 0.01 TCID50/cell. Scale bar, 100 μm. c Representative images of 2D cultured forebrain neurons exposed to an mNG-expressing clade IIb MPXV construct at a rate of 0.01 TCID50/cell. Scale bar, 100 μm. d, e Representative images acquired by transmission electron microscopy at 10 days p.i. of 95 days old hNOs infected with a clade IIb MPXV isolate with an MOI of 0.1 TCID50/cell. d’ Magnification demonstrating the presence of intracellular mature virions within cells. Scale bar, 1 µm. d” Magnification showing the presence of viral particles at different stages of development alongside perinuclear viral factories, displaying cytoskeletal condensations. Scale bar, 1 µm. e Illustration of degenerative signs, bursting and release of intracellular compartment containing high amounts of monkeypox virions. Scale bar, 1 µm.
