Fig. 4: MPXV causes neuritic beading and neuron death.
From: Monkeypox virus spreads from cell-to-cell and leads to neuronal death in human neural organoids
a, b Immunofluorescence analysis 8-14 days p.i. of 70 days old hNOs exposed to a clade IIb MPXV isolate at a rate of 0.1 TCID50/cell. a Representative micrographs showing viral antigen accumulating within regularly interspaced swellings (beads) in filaments, spanning over long distances within the tissue. DAPI, blue; MPXV, green. Scale bar, 25 µm. b Representative micrographs of beaded filaments harboring monkeypox virions co-localizing with the neuronal marker TUJ1 in both filaments and beads. White arrowheads indicate regions displaying marker co-localization. DAPI, blue; MPXV, green; TUJ1, orange. Scale bar, 10 µm. c Representative micrographs of 2D forebrain neurons exposed to an mNG-expressing clade IIb MPXV reporter construct displaying neuritic beading in filaments of a virus-harboring cell in concomitance with sudden mNG signal loss in the cell’s soma. mNG signal is subsequently observed to progressively diminish while beads still harbor detectable reporter protein signal. Images were taken at 30-min intervals, time represented in minutes. Scale bar, 100 µm.
