Fig. 5: Transcriptional profile alteration in hNOs during MPXV infection.
From: Monkeypox virus spreads from cell-to-cell and leads to neuronal death in human neural organoids
a Fold change of transcriptional expression levels of selected proinflammatory cytokine and chemokine genes including IFN-β, IL-6, IL-8/CXCL8, and IP-10/CXCL10 in 74-93 days old hNOs exposed to a MPXV clade IIb isolate at a rate of 0.1 TCID50/cell. Boxplots indicate the median value (centerline) and interquartile ranges (box edges), with whiskers extending to the lowest and the highest values. Each symbol represents an individual organoid (n = 3 independent organoid batches). Two-tailed Mann-Whitney U test was applied to compare groups. b–e Transcriptomic analysis of MPXV-challenged versus mock-treated hNOs. Organoids were infected with a MPXV clade IIb isolate at a rate of 0.1 TCID50/cell at 74–93 days of development (n = 3 independent organoid batches). hNOs were collected for RNA isolation at 4 days p.i. b PCA showing separate clustering of mock and MPXV-exposed organoids. c Volcano plot representing DEGs between MPXV-challenged and mock-treated hNOs. Two-tailed Wald test corrected for multiple comparisons using a Benjamini & Hochberg correction was applied; padj < 0.05. d GO analysis results displaying top 7 up- and downregulated biological processes in MPXV-infected versus mock-treated hNOs. One-tailed Fisher’s exact test corrected for multiple comparisons using a Benjamini Hochberg was applied; padj < 0.05. e GO analysis results displaying top 7 up- and downregulated molecular functions in MPXV-exposed and mock-treated hNOs. One-tailed Fisher’s exact test corrected for multiple comparisons using a Benjamini Hochberg was applied; padj < 0.05. Source data are provided as a Source Data file.
