Fig. 6: Tecovirimat treatment limits MPXV spread in hNOs.
From: Monkeypox virus spreads from cell-to-cell and leads to neuronal death in human neural organoids
a Representative micrographs of sectioned mock-treated (top) and MPXV-exposed hNOs cultured in regular (center) or tecovirimat-complemented medium (bottom) at 14 days p.i. Organoids were exposed to a clade IIb MPXV isolate at a rate of 0.1 TCID50/cell at 84 days of development. Tecovirimat was added to the medium 2 h after initial MPXV-exposure, to a final concentration of 1 µM. Scale bar, 2000 μm. b Representative sections of tecovirimat-treated hNOs sampled at selected time points p.i. showing unaltered tissue organization and limited virus antigen presence. Infected organoids were exposed to a clade IIb MPXV isolate at a rate of 0.1 TCID50/cell at 84 days of development. DAPI, blue; MPXV, green; TUJ1, orange; SOX2, pink. Scale bar, 1000 μm. Released (c) and cell-associated (d) infectious MPXV 2 to 14 days p.i. of 70-95 days old hNOs infected with a clade IIb isolate at a rate of 0.1 TCID50/cell and cultured in regular or tecovirimat-supplemented medium. Each symbol represents an individual organoid (n = 7-8 independent organoid batches). Unpaired, two-tailed student’s t-test was applied to compare groups. M indicates mock. Released (e) and cell-associated (f) MPXV loads detected through qPCR analysis in samples taken 2 to 14 days p.i. 84-85 days old hNOs were exposed to a clade IIb MPXV isolate at a rate of 0.1 TCID50/cell and cultured in regular or tecovirimat-supplemented medium. Each symbol represents an individual organoid (n = 3 independent organoid batches). Unpaired, two-tailed student’s t-test was applied to compare groups. M indicates mock. Dashed line indicates the detection limit of the assay(s). Source data are provided as a Source Data file.
