Fig. 5: PVHCCK-ACCGlu circuit regulates chronic visceral pain-induced changes in food preference. | Nature Communications

Fig. 5: PVHCCK-ACCGlu circuit regulates chronic visceral pain-induced changes in food preference.

From: Paraventricular hypothalamic input to anterior cingulate cortex controls food preferences in chronic visceral pain mice

Fig. 5

a Schematic of chemogenetics manipulation (left) and representative images of chemogenetic viral expression in the PVH (right). Scale bar:20 µm. b Top: Representative traces of sEPSCs in ACC neurons following bath application of ACSF and then CNO. Bottom: sEPSC peak amplitude and cumulative fraction plots (n = 7 cells/group, Pre+hM3Dq vs Post+hM3Dq, p = 0.0349; Pre+hM4Di vs Post+hM4Di, p = 0.0255). c Schematic of chemogenetic manipulation (left) and the experimental protocol of chemogenetic manipulation (right). d Heatmaps of mouse location during the free-feeding tests. e Colorectal distention thresholds before and after hM3Dq activation of PVHCCK neurons terminal in the ACC region in CON mice (n = 9 mice/group, p < 0.0001). f Food zone time percentage and food intake percentage for each liquid type before and after hM3Dq activation of PVHCCK neurons terminal in the ACC region in CON mice (n = 9 mice/group, Food intake percentage, p < 0.0001; Food zone time, IL:hM3Dq+NS vs hM3Dq+CNO, p = 0.0103, S: hM3Dq+NS vs hM3D+CNO, p = 0.0059). g Correlation between the percentage of food zone time and percentage of food intake (p = 0.0005, r = 0.5527). h CRD threshold before and after hM4Di inhibition of PVHCCK neurons terminal in the ACC region in NCI mice (n = 9 mice/group, p < 0.0001). i The percentage of food zone time and percentage of intake for each of the three liquids before and after hM4Di inhibition of the PVHCCK neurons terminating in the ACC region in NCI mice (n = 9 mice/group, Food intake percentage, p < 0.0001; Food zone time: p = 0.0002 in IL and p = 0.0007 in S). j Correlation between the percentage of food zone time and percentage of food intake (p = 0.0088, r = 0.4557). All the data are presented as the means ± SEMs. Two-sided student’s t test (b). Two-way ANOVA with Sidak’s multiple comparisons test (f, i). Ordinary one-way ANOVA with Dunnett’s multiple comparisons test (e, h). Linear regression and Correlation (g, j). *p < 0.05; **p < 0.01; ***p < 0.001.

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