Fig. 7: CCKBRs mediate the regulatory role of the PVHCCK-ACCGlu circuit in chronic visceral pain-induced food preference changes. | Nature Communications

Fig. 7: CCKBRs mediate the regulatory role of the PVHCCK-ACCGlu circuit in chronic visceral pain-induced food preference changes.

From: Paraventricular hypothalamic input to anterior cingulate cortex controls food preferences in chronic visceral pain mice

Fig. 7

a Schematic of chemogenetics manipulation and representative images of viral expression in the PVH and ACC. Scale bar:20 µm. b Representative traces of sEPSCs in ACC neurons following bath application of ACSF, CNO and then CNO with proglumide sodium. c Cumulative fraction plots and quantification of sEPSCs peak amplitude (n = 8 cells/group, Pre vs CNO, p = 0.0114; CNO vs CNO+Pro, p = 0.0121). d Schematic of chemogenetic manipulation and representative images of viral expression in the PVH and ACC. Scale bar:20 µm. e Representative traces of sEPSCs in ACC neurons following bath application of ACSF or CNO, and subsequent application of CNO with tetragastrin. f Cumulative fraction plots and quantification of sEPSCs peak amplitude (n = 6 cells/group, Pre vs CNO, p = 0.0447; CNO vs CNO+Tet, p = 0.0252). g Schematic of chemogenetic manipulation (left) and related experimental protocol (right). h Heatmaps of mouse location during the free-feeding tests. i Colorectal distension (CRD) thresholds following hM3Dq+NS, hM3Dq+CNO or hM3Dq+CNO+proglumide sodium administration to CON mice (n = 8 mice/group, p < 0.0001). j Food zone time percentage and food intake percentage following hM3Dq+NS, hM3Dq+CNO or hM3Dq+CNO+proglumide sodium administration to CON mice (n = 8 mice/group, Food intake percentage: p < 0.05; Food zone time: p < 0.05). k Correlation analysis between food zone percentage time and food intake percentage (p = 0.0107, r = 0.3652). l CRD threshold following hM4Di+NS, hM4Di+CNO or hM4Di+CNO+tetragastrin administration to NCI mice (n = 8 mice/group, p < 0.0001). m Food zone time percentage and food intake percentage following hM4Di+NS, hM4Di+CNO or hM4Di+CNO+tetragastrin administration to NCI mice (n = 8 mice/group, Food intake percentage: p < 0.05; Food zone time: p < 0.05). n Correlation between the percentage of food zone time and percentage of food intake (p = 0.0322, r = 0.3097). All the data are presented as the means ± SEMs. Two-way ANOVA with Sidak’s multiple comparisons test (j, m). Ordinary one-way with ANOVA Tukey’s multiple comparisons test (c, f, i, l). Linear regression and Correlation (k, n). *p < 0.05; **p < 0.01; ***p < 0.001.

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