Fig. 5: Cellular and tissue expression of the VLRF gene in sea lampreys. | Nature Communications

Fig. 5: Cellular and tissue expression of the VLRF gene in sea lampreys.

From: Variable lymphocyte receptor F is generated via somatic diversification and expressed by lamprey T-like cells

Fig. 5

a Expression of the VLRF gene in different lymphocyte populations in lamprey larvae. TN represents the triple-negative (VLRA−/VLRB−/VLRC−) lymphocyte population. Bars indicate the standard error of the mean in each experiment (n = 6). b Assembly status of VLRF in total RNA extracted from purified lymphocyte populations. bp, base pairs; GL, germline transcripts; M, transcripts of assembled VLRF gene. A+, VLRA+; B+, VLRB+; C+, VLRC+. Each experiment was repeated three times independently with similar results. c, d Tissue expression profiles for VLRF in larvae (c) and adult (d) lampreys. Transcripts are analyzed by real-time RT-PCR with beta-actin as a normalization control for both cellular and tissue distribution analyses. Bars indicate the standard error of the mean for three lamprey larvae (c) and three adult lampreys (d) in each experiment. Bars indicate the standard error of the mean for four lamprey larvae (c) and four adult lampreys (d) in each experiment. The corresponding data points were shown as dot plots.

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