Fig. 2: Reconstitution and cryo-EM structures of the NOT1:NOT7/8:PieF ternary complexes.
From: Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay
a Analytical size exclusion chromatography profiles (left panel) and a 15% Coomassie-stained gel (right panel) of the purified components and intermediates of the NOT1:NOT7:PieF complex. The tags used to purify the individual components were preserved during the reconstitution of the complex. Experiment was independently repeated multiple times (n > 3). Source data are provided as a Source Data file. b Mass photometry analysis of the NOT1:NOT7:PieF complex. The tags used for purification were preserved during the reconstitution of the complex. c Cryo-EM reconstruction of the NOT1:NOT7:PieF complex at 2.8 Å resolution. d Cryo-EM reconstruction of the NOT1:NOT8:PieF complex at 3.5 Å resolution. e Superimposed structures of the NOT1:NOT7/8:PieF complexes in cartoon representation. f Secondary structure annotation of PieF structure. The schematics in (a, b) were drawn using Adobe Illustrator 2025.
