Fig. 5: Recombination efficiency of pathogenic RAG1 mutants in yeast correlates with activity in mammalian cells. | Nature Communications

Fig. 5: Recombination efficiency of pathogenic RAG1 mutants in yeast correlates with activity in mammalian cells.

From: Generating combinatorial diversity via engineered V(D)J-like recombination in Saccharomyces cerevisiae

Fig. 5

a Genetic map of human RAG1 showing the core region and the location of five mutations which have been shown to cause varying degrees of immunodeficiency. Certain domains of RAG1 and their function are highlighted, including the zinc dimerization domain (ZDD) and nonamer-binding domain (NBD). b Table of human RAG1 mutants and their corresponding mutation in mouse RAG1. Additionally, the previously reported activity of the mutants in mammalian cells is given53 along with the activity measured in yeast in this work. WT = wild-type RAG1. c G418R recombination with pY112-CJA-U-H20 plasmid. BY4742 are the base strain of yeast. All other strains contain mouse RAG1core with a mutation (or without in the case of wild type, WT), RAG2, and HMGB1. Cells were plated after a 4-d induction. CFU = colony forming unit. In (c), data are presented as mean values ± SD; n = 3 biological replicates. Source data are provided in the Source Data file.

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