Fig. 2: PGC-1α modulates mitochondrial membrane structure in female BAT cells.
From: Sex difference in BAT thermogenesis depends on PGC-1α–mediated phospholipid synthesis in mice
a Representative electron micrographs of mitochondria from the BAT of Control and KO mice. The areas outlined in red boxes indicate enlarged images of mitochondria. Scale bars = 1 μm (n = 3 biologically independent experiments). b Histograms showing the distribution frequency (%) of mitochondrial section areas (0–3 µm2). c Total cristae length per mitochondrion. n = 30 per group. p = 0.2604 (Male Control vs. Male KO), p < 0.0001 (Male Control vs. Female Control), p < 0.0001 (Female Control vs. Female KO). d Representative blots for electron transport chain complexes (upper panel) and protein levels normalized to α-tubulin (lower panel); Complex I (n = 6 per group), Complex II (n = 6 Male Control, n = 6 Male KO, n = 5 Female Control, and n = 6 Female KO), Complex III (n = 6 per group), Complex IV (n = 4 Male Control, n = 6 Male KO, n = 5 Female Control, and n = 6 Female KO) and Complex V (n = 6 per group). p = 0.0014 (Complex I, Male Control vs. Female Control), p = 0.0157 (Complex I, Female Control vs. Female KO), p = 0.0275 (Complex IV, Male Control vs. Female Control). Data are expressed as the mean ± SEM. Data were analyzed by one-way ANOVA with Tukey’s post hoc test (c, d). Significance is indicated (*p < 0.05; **p < 0.01; ****p < 0.0001). ns not significant. Source data are provided as a Source Data file.
