Fig. 6: BAT-specific ChREBPβ knockdown in female mice recapitulates the metabolic phenotype of female PGC-1α knockout mice.
From: Sex difference in BAT thermogenesis depends on PGC-1α–mediated phospholipid synthesis in mice
a Gene expression of Chrebpβ and DNL-related genes in female BAT injected with adeno-associated viruses (AAV) -shScramble or AAV-shChrebp. n = 6 per group. p = 0.0014 for Chrebpβ, p = 0.0001 for Acly, p = 0.0013 for Acss2, p < 0.0001 for Fasn, p = 0.0005 for Acaca, and p = 0.1527 for Elovl6. b Gene expression of Pgc1a. n = 6 per group. p = 0.0474. c Oxygen consumption (VO2) recordings in response to NE. n = 7 per group. p = 0.0122. d Representative electron micrographs of mitochondria from BAT of AAV-Scramble and AAV-shChrebp mice. Scale bar = 1 μm (n = 3 biologically independent experiments). e Total cristae length per mitochondrion. n = 30 per group. p = 0.0271. f Percentage of CL(18:2)4 in total CL. n = 5 per group. g Percentage of ether-linked PEs in total lipids. n = 5 per group. p = 0.0236, 0.0358, and 0.0395 for PE-O(16:1/16:1), PE-O(16:1/18:1), and PE-O(18:1/16:0), respectively. h D₂O-labeled components of ether-linked PEs. n = 3 per group. p = 0.3849, 0.0376, and 0.9923 for PE-O(16:1/16:1), PE-O(16:1/18:1), and PE-O(18:1/16:0), respectively. Data are expressed as the mean ± SEM. Data were analyzed using unpaired two-sided t-tests (a, b, e), paired one-sided t-tests (f–h), and two-way repeated measures ANOVA (c). Significance is indicated (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). Source data are provided as a Source Data file.
