Fig. 4: The ubiquitin-dependent activation of SPRTN is mediated by the USD.
From: Allosteric activation of the SPRTN protease by ubiquitin maintains genome stability
a–c Indicated HMCESSRAP-DPCs (10 nM) were incubated alone or in the presence of FANCJ (100 nM) and indicated concentrations (0.1–100 nM) and variants of SPRTN (WT, L38S, L99S) for 1 h at 30 °C. Quantification: bar graphs represent the mean ± SD of three independent experiments. Source data are provided as a Source Data file.
