Fig. 3: Transcriptome analysis of osteogenic differentiation of BMSCs.
From: Self-reinforced piezoelectric chip for scaffold-free repair of critical-sized bone defects
a Volcano plots of the chip + vibration versus the film + vibration. Heatmap of osteogenesis (b), angiogenesis (c), extracellular matrix (d), and PI3K/Akt signaling pathway (e) related genes. GO (f) and KEGG (g) pathway enrichment analysis. h GSEA for the PI3K/Akt signaling pathway. i Western blotting analysis for the expression of p-PI3K and p-Akt of BMSCs in the control, control + vibration, film + vibration, and chip + vibration groups for 7 days of incubation. j Quantitative analysis of the grayscale of protein expression derived from panel (f). Data are presented as mean ± SD (n = 3 biological replicates). Two-sided ANOVA with a Tukey’s post hoc test for multiple comparisons. Source data are provided as a Source Data file.
