Fig. 4: In vitro angiogenesis.
From: Self-reinforced piezoelectric chip for scaffold-free repair of critical-sized bone defects
a Quantification of wound healing assay. b Quantitative evaluation of total branches length in tube formation assay. c Expression levels of angiogenic genes CD31 and VEGF-A of HUVECs cultured for 3 days. Semi-qualitative analysis (d) and representative immunofluorescence staining images (e) of CD31 (Red) and VEGF-A (Green) of HUVECs in different groups. Western blot results (f) and quantitative analysis (g) of protein expression of CD31 and VEGF-A. Data are presented as mean ± SD (n = 3 biological replicates). Two-sided ANOVA with a Tukey’s post hoc test for multiple comparisons. Source data are provided as a Source Data file.
