Fig. 2: Decreased ASL, increased senescence and H₂O₂ in the LepR⁺ cells of aged mice.

A–D Levels of P21, ASL, CD31, OCN, PERIPHERIN and LYVE1 in young or aged mice and quantitative results (n = 5 mice). Scale bars, 500 μm (red), 10 μm (white) and 50 μm (green). E, F Proportion of my-HSCs (Lineage⁻Sca-1⁺c-Kit⁺(LSK)CD48⁻CD150^high HSCs) in the femoral BM of young or aged mice (n = 5 mice). G Quantification of the mRNA levels of SCF (encoded by Kitl gene) in sorted LepR⁺ cell (n = 5 mice). H, I The fluorescence intensity of H₂O₂ probe in the femoral BM of young and aged mice. J H₂O₂ content in the femoral BM of young or aged mice (n = 5 mice). The data are shown as the means ± SDs. Statistical significance was determined by two-tailed unpaired Student’s t test (two groups); *P < 0.05, **P < 0.01, ***P < 0.005. Source data are provided as a Source Data file.