Fig. 3: Design of CB-NPs and CB-LepR for NO production.

A Preparation of CB-NPs. B, C Transmission electron micrographs (TEM) and diameter distributions measured by dynamic light scattering (DLS) of CB-NPs and CB-LepR, respectively. Scale bar, 200 nm. D Size stability of CB-NPs and CB-LepR detected by DLS (n = 3 independent experiments). E HPLC chromatograms of CPPO, BNN6, and CB-NPs. F, G Energy levels of BNN6 and the active DOD. H Levels of NO released after co-incubation of H₂O₂ (different concentrations) with CB-LepR. (n = 3 independent experiments) I, J Fluorescence images of LepR⁺ cells stained with an NO fluorescent probe (DAF-FM DA) after different treatments (n = 3 independent experiments). The data are shown as the means ± SDs. Statistical significance was determined by two-tailed unpaired Student’s t test (two groups) or one-way ANOVA with Dunnett’s multiple comparison tests (multiple groups); **P < 0.01, ***P < 0.005. Source data are provided as a Source Data file.