Fig. 5: NO nanopumps effectively remove H₂O₂ and generate NO.

A Schematic diagram of the detection of H₂O₂ and NO in mice subjected to different treatments. B, C Representative fluorescence images and quantitative analysis of H₂O₂ probe-treated different mice (n = 3 mice). D Intracellular levels of H₂O₂ in the BM of the different groups after 2 weeks (n = 3 mice). E Intracellular H₂O₂ levels in sorted LepR⁺ and LepR⁻ cells from different treated mice (n = 3 mice). F Intracellular NO levels in sorted LepR⁺ cells from different treated mice (n = 3 mice). G Schematic diagram of a live-cell workstation capturing NO release from CB-LepR. H Representative images of NO release from LepR⁺ cells after co-incubation with CB-LepR + H₂O₂. 3 times each experiment was repeated independently with similar results. Scale bar, 50 μm. The data are shown as the means ± SDs. Statistical significance was determined by two-tailed unpaired Student’s t test (two groups) or one-way ANOVA with Dunnett’s multiple comparison tests (multiple groups); *P < 0.05, **P < 0.01, ***P < 0.005. Source data are provided as a Source Data file.