Fig. 7: CB-LepR exhibits anti-BM microenvironment aging efficacy.

A Treatment schedule in vivo. B–F Representative micro-CT images and quantitative analysis of the bone volume per tissue volume (BV/TV), trabecular bone number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) (n = 5 mice). G–H Immunofluorescence staining for senescence, bone formation, lipid droplet formation and osteoclastic activity (n = 5 mice). Scale bars, 50 μm (red) and 10 μm (white). I Representative fluorescence images of LepR⁺ cells promoting lymphatic, vascular, and neural regeneration. Scale bar, 50 μm. J, K Proportion of my-HSCs in different treatment groups (n = 5 mice). L Heatmap of SCF (encoded by Kitl gene), Vegf-c and Ngf in the young + PBS, aged + PBS, and aged + CB-LepR groups. The data are shown as the means ± SDs. Statistical significance was determined by one-way ANOVA with Dunnett’s multiple comparison tests (multiple groups); *P < 0.05, **P < 0.01, ***P < 0.005. Source data are provided as a Source Data file.