Fig. 6: Generation of Foxp3 Timer Neonatal-to-Ageing Benchmarking Data across developmental and ageing stages in WT Foxp3-Tocky mice.
From: Machine learning-assisted decoding of temporal transcriptional dynamics via fluorescent timer
a Timer Blue and Timer Red expression in CD4 + T cells from the thymus and spleen of WT Foxp3 Timer mice at various ages. All samples within the training dataset were concatenated per group and shown as pseudocolour plots. Days and weeks since birth are indicated. b Normalised Timer fluorescence data from the flow cytometric analysis in a. c Timer Angle and Intensity transformed from the normalised Timer fluorescence data in b. d Percentage of CD4+ T cells in each of the indicated gates or the mean Timer Angle. e Percentage of mean Timer Angle plotted against logarithmically transformed age, with axis labels indicating actual age in days. Each line represents a quadratic regression model for each organ’s data. Shaded areas indicate 95% confidence intervals around the fitted regression lines.
