Fig. 4: In vitro therapeutic effects in osteosarcoma eradication. | Nature Communications

Fig. 4: In vitro therapeutic effects in osteosarcoma eradication.

From: Sono-activable and biocatalytic 3D-printed scaffolds for intelligently sequential therapies in osteosarcoma eradication and defect regeneration

Fig. 4

a Schematic illustration of the therapeutic mechanism of HS-ICTO under TME condition. b POD-like activity by evaluating the TMB absorbance value at λ = 652 nm after incubation with HS-ICTO-x (n = 3 independent experiments, data are presented as mean ± SD). c Sono-activable biocatalytic oxidation of TMB and DPA under the co-incubation of HS-ICTO (n = 3 independent experiments, data are presented as mean ± SD). d PCA plot for different cellular samples. e Volcano plots showing differential gene expression in HS-ICTO + US vs. HS and HS-ICTO vs. HS comparison (Sig up, significantly upregulated; Sig down, significantly downregulated; Up, upregulated; Down, downregulated; Non-Sig, nonsignificance). f GO enrichment analysis of the differentially expressed genes associated with ROS-triggered damage, stress response, and apoptosis biological process in 143b cells. The data in (d–f) were representative of three biologically independent samples from each group. g Representative CLSM images of the live/dead staining of 143b cells on the scaffolds after different therapies. The images were representative of three independently repeated experiments from each group. h Flow cytometric apoptosis analysis of Annexin V-FITC/PI-stained 143b cells harvested from scaffolds with different treatments. The gate strategy is shown in Supplementary Fig. 53. The plots were representative of three independently repeated experiments from each group. i Expression of HIF-1α and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in 143b cells harvested from different scaffolds analyzed by western blot assay (n = 3 biologically independent replicates). The assessment in (c) of P-values was performed by a two-tailed Student’s t-test. In (e), P-values were obtained from two-sided DESeq2 test without multiple comparison. In (f), P-values were obtained from one-sided Hypergeometric test without multiple comparisons. In (b, c), a.u. indicates the arbitrary units. Source data are provided as a Source Data file.

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