Fig. 7: Downregulation of Man-PTS EII increases CPS biosynthesis via the cAMP-CRP regulon. | Nature Communications

Fig. 7: Downregulation of Man-PTS EII increases CPS biosynthesis via the cAMP-CRP regulon.

From: Lactate promotes invasive Klebsiella pneumoniae liver abscess syndrome by increasing capsular polysaccharide biosynthesis via the PTS-CRP axis

Fig. 7

a Intracellular cAMP concentration in the wild-type (WT), Δman-PTS EII mutant, and complemented mutant (C-Δman-PTS EII) strains cultured in LB or Lac-LB (n = 3). b CPS quantification of the 10 clinical K. pneumoniae isolates grown in LB broth supplemented with lactate and/or cAMP. c HMV of the same 10 isolates cultured in Lac-LB with or without cAMP supplementation. d CPS quantification of the WT and Δcrp strains in LB or Lac-LB (n = 3). e Transcriptional expression of CPS gene cluster in the WT and Δcrp strains cultured in LB or Lac-LB (n = 3). the isolates used in panels b and c are the same as those shown in Fig. 3a. n indicates the number of biological replicates for all experiments. Statistical analysis in (c) was performed using a two-sided independent-samples t-test, while other panels were analyzed by one-way ANOVA with Tukey’s post-tests. In panels (a, d, e), error bars show the mean ± SD. In panels (b, c), Each point represents the mean value of three biological replicates for each strain. Source data are provided as a Source Data file.

Back to article page