Fig. 4: AP Profiling resolves chaperone effects on co-translational folding. | Nature Communications

Fig. 4: AP Profiling resolves chaperone effects on co-translational folding.

From: Arrest Peptide Profiling resolves co-translational folding pathways and chaperone interactions in vivo

Fig. 4

a Trigger factor and DnaK compete for interactions with nascent chains on the ribosome. b Ablation of trigger factor (\(\Delta\) tig, gold) resulted in localized changes of AP scores compared to a wild-type background (WT, black). c Same as panel (b) for a DnaK deletion strain (\(\Delta\) dnaKJ, blue). d Δchaperone scores, obtained as the difference in AP scores between wild-type and chaperone-knockout strains, exhibit the most prominent change in the region of intermediate folding for both \(\Delta\) tig and \(\Delta\) dnaKJ. e Δchaperone scores mapped onto the GEF-Tu structure illustrate localization of chaperone deletion effects at distinct sites within the same region (around amino acid 200). f Chaperone deletion has only minor effects on GEF-G scores, compared to GEF-Tu. Source data are provided as a Source Data file.

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