Fig. 5: Neutrophil gelatinase-associated lipocalin (NGAL) level is increased in colon of mice with chronic colitis and in aldosterone-induced colon fibroblasts. | Nature Communications

Fig. 5: Neutrophil gelatinase-associated lipocalin (NGAL) level is increased in colon of mice with chronic colitis and in aldosterone-induced colon fibroblasts.

From: Mineralocorticoid receptor activation contributes to intestinal fibrosis through neutrophil gelatinase-associated lipocalin in preclinical models

Fig. 5

a, b Male C57BL/6 J mice underwent 3 cycles of 2% dextran sulfate sodium (DSS) in their drinking water for 7 days, followed by 14 days of regular water (DSS, n = 11) for both first cycles and 7 days for the third cycle. whereas control mice received normal water (CT, n = 10). Mice were subjected to either a standard diet or diet supplemented with spironolactone (30 mg.kg-1; DSS+Sp, n = 12) throughout the experiment. a Relative mRNA and b protein expression of NGAL from colon of CT, DSS and DSS+Sp mice (n = 10 for CT, n = 11 for DSS, n = 12 for DSS+Sp; Kruskal-Wallis test with Dunn’s multiple comparisons test for mRNA expression; ordinary one way ANOVA with Tukey’s multiple comparisons test for protein expression). c–d Female C57BL/6 J mice underwent 3 cycles of 2% DSS in their drinking water for 7 days, followed by 14 days of regular water (DSS-WT, n = 8; DSS-sm22MR−/−, n = 10) for both first cycles and 7 days for the third cycle. c Relative mRNA and d protein expression of NGAL from colon of CT-WT, DSS-WT, DSS-sm22MR−/−mice (n = 8 for DSS = WT, n = 10 for DSS-sm22MR−/−; Two-sided unpaired t-test with Welch’s correction for mRNA epression; Two-sided Mann-Whitney test for protein expression). e Colon immunostaining of NR3C2, NGAL and SGK1 (n = 5 for CT, n = 10 for DSS, n = 12 for DSS+Sp; Kruskal-Wallis test with Dunn’s multiple comparisons test). f CCD-18Co cells were incubated with aldosterone (100 nM), spironolactone (10 µM) in response to TGF-β (10 ng/mL) for 24 h (n = 5 from independent experiments). Relative protein expression of NGAL from CCD-18Co cells lysates (n = 5 per group; ordinary one way ANOVA with Tukey’s multiple comparisons test). Data are presented as mean values +/− SEM.

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