Fig. 4: High avidity interaction with target cells drives CAR-T proliferation and sustained effector function. | Nature Communications

Fig. 4: High avidity interaction with target cells drives CAR-T proliferation and sustained effector function.

From: Functional avidity of anti-B7H3 CAR-T constructs predicts antigen density thresholds for triggering effector function

Fig. 4

a, b CAR-T cells stimulated with irradiated LAN-1 or Kelly neuroblastoma cells at a 1:1 E:T ratio every 7 days; IFN-γ (a) and IL-2 (b) measured in 24 h post stimulation supernatants (mean ± SEM, N = 3 independent donors, two-way ANOVA). c T cell numbers in same cocultures evaluated using precision count beads and flow cytometry (mean ± SEM, N = 3 independent donors, two-way ANOVA). d T cell counts were performed using the same precision count bead methodology in 72 h low E:T ratio LAN-1 and SK-N-SH cocultures, cytotoxicity for which is shown in Fig. 3c (mean ± SEM, N = 3 independent donors, two-way ANOVA: for LAN1 376 vs MGA 0.0168, TE9 vs MGA p = 0.0036). e, f Relative T cell proportions measured using flow cytometry in 691-B and 691-T tumor cocultures over 3 weeks of weekly tumor challenges at an E:T ratio of 1:10. Gated on single cells of harvested cultures (T cells and tumor cells) at days 14 and 21 and gated on all cells at day 0 prior to targets being added, representative dot plots from one donor CAR-T are shown. The numbers shown on the plots are indicative of % live tumor and % live T cells within each culture of total cells.

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