Fig. 5: High avidity interactions drive CAR-T proliferation and cytotoxicity in brain tumor models. | Nature Communications

Fig. 5: High avidity interactions drive CAR-T proliferation and cytotoxicity in brain tumor models.

From: Functional avidity of anti-B7H3 CAR-T constructs predicts antigen density thresholds for triggering effector function

Fig. 5

a B7H3 molecules per cell were quantified using QuantiBright flow cytometry assay; brain tumor targets in blue, neuroblastoma and rhabdoid tumor targets in gray. b T cell proliferation in response to live brain tumor cell challenge at a 1:1 E:T ratio quantified after 6 days of co-culture (mean ± SEM, three independent T cell donors across two experimental replicates, Kruskal–Wallis test). c T cell fold-expansion plotted against number of B7H3 molecules per target cell; symbols represent mean proliferation of the different donors and replicates; lines are simple linear regression curves. d Mean fold-proliferation of CAR-T cells against Med8a and LAN-1 cells plotted against mean avidity at 1000 pN for the two cell lines. Symbols represent the mean proliferation of the different donors and replicates; the lines are simple linear regression curves.

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