Fig. 1: Differentiated intestinal epithelial cells display star-shaped actin cytoskeleton.

A Scheme showing the functional organization of the mouse small intestinal tissue where the proliferative crypt domains are shown in blue and the differentiated villus compartment in yellow. Created in BioRender. Delacour, E. (2025) https://BioRender.com/phiefj6. Ba–d Confocal analysis of actin distribution in the villus domain. Nuclei are shown in blue in (d). Green arrowheads point to actin stars (AcSs). Scale bars, a 50 μm, b–d 10 μm. N = 3 mice, n (villi) = 20. C Scheme showing the self-organization of intestinal organoid-derived monolayer with the proliferative crypt-like domains in blue and the differentiated villus-like compartment in yellow. Created in BioRender. Delacour, E. (2025) https://BioRender.com/9q40qag. Da–d Confocal analysis of actin distribution in the apical or basal side of an organoid-derived monolayer. xz view is presented in (d). Crypt-like domains are delimited in blue. Green arrowheads point to actin stars. C, crypt-like domain. Scale bars, a, b 100 μm, c 10 μm, d 50 μm. N = 30 monolayers.