Fig. 1: Comparison of sequencing depth, richness, and viral community composition between negative controls and samples.
From: Negativeome characterization and decontamination in early-life virome studies
a Number of clean reads in negative controls (NCs) vs samples. b Viral operational taxonomic unit (vOTU) richness in NCs vs samples. In (a, b), data are shown for 1313 samples and 55 NCs, distributed by study as follows: Garmaeva et al. (NCs = 1, samples = 205); Liang et al. (NCs = 38, samples = 383); Maqsood et al. (NCs = 8, samples = 78); Shah et al. (NCs = 8, samples = 647). c, d Non-metric multidimensional scaling analysis utilizing Bray–Curtis dissimilarity, computed at: c the vOTU level; d predicted host level. In (c), data are shown for 33 NCs and 1228 samples: infants <5 months (n = 372), infants >5 months (n = 710), mothers (n = 146). In (d), data are shown for 25 NCs and 1147 samples: infants <5 months (n = 290), infants >5 months (n = 711), and mothers (n = 146). For (c and d), a list of outliers not depicted in the plots can be found in Supplementary Data 13. In (a–d), every dot is a sample, and the dot color indicates age: infant samples (age <5 months) in yellow, infant samples (age > 5 months) in orange, maternal samples in red, and NCs in dark blue. In (a–d), boxplots visualize the median, hinges (25th and 75th percentiles), and whiskers extending up to 1.5 times the interquartile range from the hinges. Asterisks denote Benjamini–Hochberg-adjusted statistical significance values: *FDR   <   0.050, ***FDR   <   0.001, ns not significant. ‘NA’ is used when significance cannot be calculated.
