Fig. 2: Confined and non-confined states of the cholesterol probe and toxin-labelled nAChR trajectories and their corresponding diffusive behaviour.

a Representative single tracks of CF®640R-BTX-labelled nAChR under control conditions showing parts classified as confinement sojourns (green) and as free, non-confined portions (black). Scale bar = 100 nm. b Boxplots showing the mean and SEM of lifetimes of non-confined portions (left) and confinement sojourns (second left), confined ratio r (second right, defined as before r = confined portion lifetimes / total trajectory time), and transition rates between confined and non-confined states of the trajectories of CF®640R-BTX (19,427 confined portions, 19,443 non-confined portions, and 1383 trajectories in 16 ROIs), CDx (9169 confined portions, 9099 non-confined portions, and 853 trajectories in 15 ROIs), CF®680R-BTX (2458 confined portions, 33,064 non-confined portions, and 187 trajectories in 5 ROIs), CF®680R-BTX (+fPEG-Chol) (32,881 confined portions, 21,923 non-confined portions, and 1373 trajectories in 47 ROIs), fPEG-Chol (18,210 confined portions, 18,300 non-confined portions, and 1012 trajectories in 6 ROIs), and fPEG-Chol (+CF®680R-BTX) (161,601 confined portions, 21,923 non-confined portions, and 5229 trajectories in 52 ROIs). Each grey dot represents a trajectory inside an ROI, and the coloured dots represent the average of the parameter for a given ROI. c Boxplots showing the mean and SEM of the percentage of trajectories in each experimental ROI classified as subdiffusive (β < 0.9), Brownian (0.9 ≤ β ≤ 1.1), and superdiffusive (β > 1.1) in confinement sojourns (upper panels) and non-confined portions (lower panels), respectively, upon TA-MSD fitting up to 25 ms for the different sample conditions as labelled. Source data are provided as a Source Data file.