Fig. 3: Abundance proteomics analysis of pediatric TB cohorts.

a Benchmark of data between patients with respiratory burden and healthy controls, excluding Latent TB Infection. X-axis shows the TB classification status, while y-axis represents the protein-level intensity. Box shows the protein intensities for individual samples (dots), the median value (center line) IQR range (box limits), and 1.5 times the IQR (whiskers). P-values are calculated from a two-sided Kruskal–Wallis test. N-values represent the number of patients within each group. b Volcano plot between Confirmed (n = 133) and Unlikely TB (n = 231). The x-axis shows the Log2 fold change at the protein level, while the y-axis represents the significance as −log10 of the Benjamini–Hochberg (BH) corrected p-values derived from a two-sided Welch t-test. Significant proteins (BH-adjusted p < 5%) are shown in red (upregulated) and blue (downregulated). Yellow dots indicate inflammatory marker proteins from (a). Barplot showing the number of differentially expressed proteins (DEPs) that were either upregulated (red, n = 17) or downregulated (blue, n = 30). c Density plot showing the z-scored intensity for the most significantly regulated protein (IGHV3-30), divided by TB status in confirmed TB (pink), unconfirmed TB (green), and unlikely TB (blue). d Gene set enrichment analysis for identification of dysregulated pathways between Confirmed TB and Unlikely TB. Dot size represents the BH adjusted p from a two-sided mean difference (MD) test of protein abundances. Colors indicate the overlap between each signaling pathway and the protein dataset. Only pathways with over 60% overlap are represented. Source data are provided as a Source Data file.