Fig. 4: 3D-PAULM of AAV-Cre mediated BphP1 expression in liver and spleen. | Nature Communications

Fig. 4: 3D-PAULM of AAV-Cre mediated BphP1 expression in liver and spleen.

From: Deep-tissue high-sensitivity multimodal imaging and optogenetic manipulation enabled by biliverdin reductase knockout

Fig. 4

a Schematic of Cre-mediated tissue-specific expression of BphP1 in loxP-BphP1 mice, and the wide-field fluorescence images of the AAV-Cre-mediated BphP1 expression in the liver of Blvra−/− and Blvra+/+ mice. The signals outside the liver region are mostly autofluorescence signals from the animal fur. Created in BioRender77. b 3D-PAULM images of the BphP1 expression in the liver of the Blvra−/− mouse. Two projections along the x-y and y-z planes are presented. c Wide-field fluorescence images of the BphP1 expression in the spleen of loxP-BphP1 Blvra−/− and loxP-BphP1 Blvra+/+ mice. Created in BioRender77. d 3D-PAULM projection images of the BphP1 expression in the spleen of the loxP-BphP1 Blvra−/− mouse. e 3D-PAULM volumetric rendering of the BphP1 expression in the liver of the loxP-BphP1 Blvra−/− mouse. f Contrast-to-noise ratio of BphP1 signals in the Blvra−/− and Blvra+/+ liver and spleen. Data are presented as mean values +/- SD (n = 3 independent injection sites). An unpaired (parametric, two-tailed) Student’s t-test, p = 0.0134 for the liver and p = 0.0442 for the spleen. Source data are provided as a Source Data file.

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