Fig. 1: Evaluation of triple negative breast cancer cell line MDA-MB-231 with increased DVL1 expression reveals numerous differentially expressed genes (DEGs) and core promoter binding.
From: Dishevelled-1 regulates global transcriptomic changes and associates with ETS1 transcription factor
a Differential gene expression analysis of RNA sequencing of MDA-MB-231 with increased expression of DVL1 (DVL1OE) in comparison with control cells (EV). Number of significantly expressed (n = 3 biological replicates, log2FC > 2 or < − 2, padj < 0.05, statistical analysis and p-value calculations were performed using EdgeR) genes after TREAT, Top Right; Categorical placement of genes to identify gene family type, Bottom Right (b) RT-qPCR (n = 3 biological replicates (3 technical replicates each), mean ± SD, two-way, ANOVA followed by Sidak’s multiple comparison test was used to test significance) of APCS, MMP1, BMP2, CXCR4, SIRPA, TNFRSF18 in MDA-MB-231 control cells (EV) and 231 cells with increased DVL1 expression (DVL1OE), and HEK293TREx DVL triple knock out (DVL-/-/-) control cells and HEK293TREx DVL-TKO with increased DVL1 expression (DVL+/-/-). c Visualization of DVL1 (D3570, 27384-1-AP), and negative control genomic region binding peaks in IGV in intronic regions, 3’UTR, 5’UTR and intergenic regions, overlayed with RefSeq, and markers for H3K27ac, H3K4me2, H3K27me3, H3K4me1, H3K9ac, H3K36me3, and POL2RA. Source data are provided as the Source Data file.
