Fig. 2: Comparison of continuous processing of severe respiratory syndrome coronavirus 2 (SARS-CoV-2) non-structural protein 7-11 C (nsp7 11 C) and nsp7-11N.
18 µM nsp7-11 with N- or C-terminal His6-tag were mixed with 3 µM main protease (Mpro) at 27 °C. Standard errors were calculated from triplicates. A Representative mass spectra of nsp7-11C showing continuous in-capillary processing at 2 min, 14 min and 28 min. B Decays of intermediate species of nsp7-11C and nsp7-11N are plotted and dominated by nsp7-11C/nsp7-11N (blue) and nsp7-10/nsp7-10N species (turquoise). Data points were simply connected for enhanced visibility. C Decline of cleavage site 10/11 (CS10/11) and CS9/10 for nsp7-11C and nsp7-11N were plotted on a logarithmic scale, and data points were fitted for the linear region using first-order kinetics. Source data are provided as a Source Data file.
