Fig. 3: Discrete processing approach of severe respiratory syndrome coronavirus 2 (SARS-CoV-2) non-structural protein 7-11 (nsp7-11).
19 µM nsp7-11 was mixed with 3.5 µM Mpro at 0 °C. A discrete processing approach was conducted in triplicate measurements. Error bars are standard error. A Representative mass spectra at different time points. B Course of the individual intermediate species of the polyprotein assigned to the four corresponding cleavage sites (CS) (indicated on the top right). Data points are connected for better visibility. C Determination of the rate constants k by following the depletion of the substrates corresponding to each cleavage site. The decay is represented as a fitted line. Source data are provided as a Source Data file.
