Fig. 2: Higher frequency of CD4⁺ regulatory T (Treg) cells post-partum with increasing number of pregnancies.

a Experimental setup: age-matched C57Bl/6 J mice were allogenically mated to Balb/c males once, twice or three times, respectively, and compared to virgin mice at the age of 21 weeks. b–j Flow cytometry analysis was performed 4 weeks after the last delivery including frequencies of CD4⁺ Treg cells and associated CD44^high expression in (b) PBMCs (virgin: n = 5; 1^st: n = 5, 2^nd: n = 4, 3^rd: n = 5), c–f lymph node (virgin: n = 10 (4); 1^st: n = 5, 2^nd: n = 4, 3^rd: n = 5; c virgin vs 2^nd: p = 0.0277, virgin vs 3^rd: p = 0.0078; e virgin vs 3^rd: p = 0.0388) and (g–j) uterus (virgin: n = 4; 1^st: n = 5, 2^nd: n = 4, 3^rd: n = 5; g virgin vs 3^rd: p = 0.0194), respectively. Additional plots are shown in Supplementary Fig. 2. Data are presented as violin plots with individual point and median and quartiles. The statistical significance was calculated using One-way-Anova (* p < 0.05, ** p < 0.01). Source data are provided as a Source Data file.